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The wonderful world of suspension cells: why this culture method has changed science.
On the stage of scientific research, suspension cell culture technology is like a silent but indispensable actor, playing a key role in many important biomedical advances. This culture method, by enabling cells to grow and multiply without attaching to a surface, offers researchers new possibilities, particularly in areas such as antibody production, vaccine development and cell biology.
Suspension cell culture technology not only simplifies many experimental steps, but also makes large-scale cell production possible.
Historical Background of Suspension Cell Culture
The history of suspension cell culture is closely tied to the history of cell culture, dating back to 1885, when Wilhelm Roux created a physiological salt solution to maintain living cells (such as chicken embryos) . With the development of technology, Ross Granville Harrison explored the in vitro cell culture of nerve cells in 1907, which marked the birth of cell culture technology. In 1910, Montrose Thomas Burrows and Alexis Carrel collaborated to develop the technique of multiplex tissue culture, which allowed it to be maintained in vitro, a breakthrough. .
In 1952, George Otto Gay and his assistant Mary Kubicek cultured the first human-derived immortalized cell line, HeLa cells, which are suspended A major milestone in cell culture.
Culture methods and maintenance of suspension cells
The culture method of suspension cells is simpler than that of attached cells. All primary cells must first be removed from the organism and dissociated, then suspended in culture medium for cultivation. Normally, white blood cells can be efficiently cultured in a suspended state because they are naturally present in the blood. However, most mammalian cells need to attach to a surface in order to divide, so suspension culture is not a suitable option for these cells.
During suspension cell culture, it is critical to keep the cells in suspension, which usually requires specific equipment such as magnetic stirrers and shakers.
Commercial Applications and Future Potential
The commercial applications of suspension cell cultures are extremely broad. Its ability to grow large numbers of cells in smaller containers makes it irreplaceable in the large-scale production of antibodies, therapeutic proteins and other important biologics. For example, antibodies produced using suspension cell culture techniques are now widely used in cancer treatment. Suspension cell culture also shows excellent results in yeast fermentation and recombinant protein production.
ConclusionInnovations in suspension cell culture technology are enabling more efficient production of vaccines and medicines, which could have a significant impact on global public health.
Suspension cell culture has not only revolutionized the application of cell culture technology, but also opened up unlimited possibilities for the future of biomedical research. The development of this technology is definitely worth our continued attention. As science advances, how will the potential of suspension cell culture continue to change our world?
