A point-of-care test of active matrix metalloproteinase-8 (Ammp-8) predicts triggering receptor expressed on myeloid cells-1 (TREM-1) levels in saliva.

Abstract

BACKGROUND\nThis cross-sectional study aims to investigate if a point-of-care (PoC) test of active matrix metalloproteinase-8 (aMMP-8) predicts levels of inflammation amplifier TREM-1 and its putative ligand the neutrophil peptidoglycan recognition protein 1 (PGLYRP1) in saliva.\n\n\nMETHODS\nForty-seven adolescents, aged 15-17, were tested with aMMP-8 PoC test, which was followed by a full-mouth clinical examination of the assessment of periodontal, mucosal and oral health. TREM-1 and PGLYRP1 levels were analyzed by ELISA. The IFMA assay specific for aMMP-8 was used as the reference method.\n\n\nRESULTS\n14 saliva samples out of total of 47 samples showed positivity for aMMP-8 PoC test. Both the TREM-1 and the aMMP-8 (IFMA) levels were significantly elevated among the aMMP-8 PoC test positives compared to the PoC test negatives (P<0.05). Moreover, aMMP-8 levels assessed by IFMA showed a strong positive correlation with TREM-1 levels in saliva (r = 0.777, P < 0.001). The number of periodontal pockets with ≥4mm was significantly lower among the adolescents with a negative aMMP-8 PoC test result and TREM-1 levels below 75 pg/mL (P<0.05). In contrast, adolescents with a positive aMMP-8 PoC test result (i.e. elevated aMMP-8 levels) together with elevated TREM-1 levels had significantly higher number of periodontal pockets with ≥4mm (P<0.001).\n\n\nCONCLUSIONS\nThe present study validated usability of aMMP-8 PoC test for predicting pro-inflammatory salivary profile and periodontal health status in adolescents. This article is protected by copyright. All rights reserved.