A review of bioanalytical methods for chronic lymphocytic leukemia drugs and metabolites in biological matrices.

Abstract

Quantitation of drugs being used for the treatment of chronic lymphocytic leukemia (CLL) in various biological matrices during both pre-clinical and clinical development is very important and often in routine therapeutic drug monitoring. The first developed methods for quantitation was traditionally done on liquid chromatography (LC) in combination with either ultra violet or fluorescence detection. However, the emergence of LC with mass spectrometry in tandem in early 1990s has revolutionized the quantitation as it has provided better sensitivity and selectivity within a shorter run time, hence it became choice of method for the analysis of various drugs. In this review, an overview of reported various bioanalytical methods (HPLC or LC-MS/MS) for the quantification of drugs for the treatment of CLL is given along with applicability of these methods is critically discussed.