Nerve regeneration in rat peripheral nerve allografts: Evaluation of cold‐inducible RNA‐binding protein in nerve storage and regeneration

Abstract

The prevalence of peripheral nerve injury has attracted increased attention. Allografting has been proposed as a potential treatment strategy for peripheral nerve injury. Moreover, cryopreservation may provide almost unlimited graft material. We investigated whether cold‐inducible RNA‐binding protein (CIRP) could protect peripheral nerves during cryopreservation to promote regeneration postoperation. First, CIRP was highly expressed after pretreatment at 32°C. After 4\u2009weeks of cryopreservation, the increased live cells, low Bax/Bcl‐2 ratio and high nerve growth factor and glial cell‐derived neurotrophic factor levels in the 32°C group demonstrated high nerve graft viability. At 4\u2009weeks postoperation, 32°C‐Allo group demonstrated low plasma levels of interleukin‐6 and interferon‐gamma and a diminished cellular immune response. At 20\u2009weeks postoperation, nerve regeneration in the 32°C‐Allo group was similar to that in the fresh isograft group and superior to that in the 4°C‐Allo and 15°C‐Allo groups. Moreover, the compound muscle action potential and the motor nerve conduction velocity of the 32°C‐Allo group were equal to those of the fresh isograft group. In conclusion, CIRP induction increased Schwann cell biological activity, inhibited cell apoptosis, reduced immune rejection, and promoted recipient nerve regeneration. Thus, CIRP could exert protective effects during nerve storage and stimulate regeneration in peripheral nerve reconstruction.