Single cell capture, isolation, and long‐term in‐situ imaging using quantitative self‐interference spectroscopy

Abstract

Single cell research with microfluidic chip is of vital importance in biomedical studies and clinical medicine. Simultaneous microfluidic cell manipulations and long‐term cell monitoring needs further investigations due to the lack of label‐free quantitative imaging techniques and systems. In this work, single cell capture, isolation and long‐term in‐situ monitoring was realized with a microfluidic cell chip, compact cell incubator and quantitative self‐interference spectroscopy. The proposed imaging method could obtain quantitative and dynamic refractive index distribution in living cells. And the designed microfluidic chip could capture and isolate single cells. The customized incubator could support cell growth conditions when single cell was captured in microfluidic chip. According to the results, single cells could be trapped, transferred and pushed into the culture chamber with the microfluidic chip. The incubator could culture single cells in the chip for 120\u2009h. The refractive index sensitivity of the proposed quantitative imaging method was 0.0282 and the relative error was merely 0.04%.