Potential false‐positive reasons for SARS‐CoV‐2 antibody testing and its solution

Abstract

Coronavirus disease 2019 (COVID‐19) has brought a huge impact on global health and the economy. Early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection is essential for epidemic prevention and control. The detection of SARS‐CoV‐2 antibodies is an important criterion for diagnosing COVID‐19. However, SARS‐CoV‐2 antibody testing also has certain false positives causing confusion in clinical diagnosis. This article summarizes the causes of false‐positive detection of SARS‐CoV‐2 antibodies in clinical practice. The results indicate that the most common endogenous interferences include rheumatoid factor, heterophile antibodies, human anti‐animal antibodies, lysozyme, complement, and cross‐antigens. The exogenous interference is mainly incomplete coagulation of the specimen, contamination of the specimen, and insufficient optimization of the diagnostic kit s reaction system.