Preparation, evaluation and application of dummy molecularly imprinted polymer for analysis of hesperidin in lime juice.

Abstract

Lime juice as the most commonly used natural source production, can be characterized using determination of flavonoids contents such as hesperidin. So, development of analyzing methods for checking the quality and healthiness of lime juices is necessary. In this study, we aimed to set up a selective solid phase extraction method using dummy molecularly imprinting approach for extraction and separation of hesperidin in lime juice to check the quality of commercial lime juice products of Mashhad city market. The imprinted polymers were synthesized by hesperitin as dummy template due to the hesperitin solubility in the wide range of porogenic solvents. The specificity extent of synthesized polymers toward hesperidin were tested and optimum one was used as adsorbent in solid phase extraction cartridge. The dummy molecularly imprinted polymer with high adsorption capacity for hesperidin (dissociation constant 0.12 μM) was successfully used for extraction and clean-up of hesperidin in the lime juice matrix prior to analysis by HPLC. The analysis of hesperidin was done in the range of 0.312-50 μg/mL with detection limit of 0.05 μg/mL. This technique was successfully set up to remove the interfering compounds for analysis of hesperidin in commercial lime juice products. This article is protected by copyright. All rights reserved.