Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium pressure liquid chromatography and reversed phase liquid chromatography.

Abstract

Saxifraga tangutica is widely used as a medicinal herb to treat hepatic diseases. Here, we developed a class separation method to separate of gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from the methanol extract of Saxifraga tangutica. Firstly, an MCI GEL® CHP20P medium-pressure liquid chromatography was used to pretreat of the crude extract from Saxifraga tangutica (500 g) and the target sample (fraction Fr1, 1.7 g) was obtained. Then, an on-line reversed phase liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay was employed for recognizing potential 1,1-diphenyl-2-picrylhydrazyl inhibitors and six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were recognized from fraction Fr1. Subsequently, the six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were isolated via a ReproSil-Pur C18 AQ preparative column. During the separation process, the hydrophilic liquid chromatography was used to enrich the target compounds (Fr1-3-1-1 and Fr1-3-1-2) from the fraction Fr1-3, which were hardly isolated only by one step reversed phase liquid chromatography. Finally, six gallic acid derivatives were obtained and identified as gallic acid (Fr1-1-1), gallic acid 3-O-β-D-glucoside (Fr1-1-2), protocatechuic acid (Fr1-2), 4-O-galloyl-(-)-shikimic acid (Fr1-3-1-1), 5-O-galloyl-(-)-shikimic acid (Fr1-3-1-2) and 3-O-galloyl-shikimic acid (Fr1-4), respectively. Thus, the present study indicated that this method was highly efficient for the preparative separation of gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from natural products. This article is protected by copyright. All rights reserved.