Extraction and determination of tussilagone from Farfarae Flos with online solid-phase extraction-HPLC using a homemade monolithic cartridge doped with porous organic cage material.

Abstract

A solid-phase extraction cartridge was fabricated using diallyl isophthalate as the monomer with the addition of porous organic cage material via in situ free radical polymerization in a stainless-steel column. The resulting monolithic adsorbent exhibited a relatively uniform porous structure, a high specific surface area of 113.98 m2 g-1 , and multiple functional chemical groups according to the characterization results. An online solid-phase extraction-HPLC procedure was fabricated to extract and determine tussilagone from Farfarae Flos. The results show that the complex sample matrices can be removed in the solid-phase extraction procedure; simultaneously, tussilagone can remain, which can be subsequently switched to an octadecylsilane bonded\xa0analytical column. The methodological validation showed that the correlation coefficient was 0.9999 with a linear range of 0.6∼200.0 μg mL-1 , the limit of detection was 0.2 μg mL-1 , the limit of quantification was 0.6 μg mL-1 , the accuracy was 100.3∼100.6%, and the relative standard deviation of precision was ≤ 1.9%. The present monolithic cartridge exhibits good reusability of no more than 100 times. The real sample of Farfarae Flos was determined with a tussilagone content of 0.74 mg g-1 . This article is protected by copyright. All rights reserved.