Cell Tracking of Chromium-labeled Mesenchymal Stem Cells using Laser Ablation Inductively Coupled Plasma-Imaging Mass Spectrometry.

Abstract

RATIIONALE\nMesenchymal stem cells (MSCs) are widely used in regenerative medicine research. Evaluating the biodistribution of MSCs is important for determining whether the cells have reached the target tissue, and the time that the stem cells reside in each area is required to estimate the duration of efficacy.\n\n\nMETHODS\nA laser ablation inductively coupled plasma-imaging mass spectrometry (LAICP-IMS) method was developed for highly sensitive and quantitative surface analysis of metal elements for solid samples. We evaluated the usefulness of a cell-tracking system with LAICP-IMS to investigate the biodistribution of mouse mesenchymal stem cells (mMSCs) labeled with the natural composition of chromium (Cr) in mice. To prepare the dosing solution, mMSCs were incubated with both Na2 CrO4 and fluorescent labeling solutions. The concentration of the cells was adjusted by vehicle solution at 2.0 to 2.5 × 107 cells/mL, and the dosing suspension of mMSCs was administered by intramuscular or intravenous injection to the mice.\n\n\nRESULTS\nThigh muscle sections after intramuscular injection of 52 Cr- and fluorescence-labeled mMSCs were analyzed by LAICP-IMS and fluorescence microscopy, respectively. 52 Cr+ mass spectrometry and fluorescence signals were detected in the same thigh muscle sections after mMSC administration A half-body section was also analyzed by LAICP-IMS. 52 Cr+ signals were mainly detected in the lungs.\n\n\nCONCLUSIONS\nThe 52 Cr+ signals were observed in sections through the thigh muscle and half body after intramuscular and intravenous administration, respectively, of Cr-labeled mMSCs to mice. Our results suggest that LAICP-IMS is sensitive and useful technique to evaluate biodistribution in the cell therapy research.