Methods in molecular biology | 2019
Generation of Skeletal Myocytes from Embryonic Stem Cells Through Nuclear Receptor Signaling.
Abstract
The differentiation of mouse embryonic stem (ES) cells into skeletal myocytes can be enhanced by small molecular inducers, which signal through muscle-specific transcription networks. However, to induce differentiation in vitro, the ES cells must be thrusted through the stage of embryoid body (EB) formation. Here, we describe how to efficiently direct the commitment of ES cells into skeletal muscle lineage by using nuclear receptor agonists in a hanging drop protocol.