Archive | 2021
Relevance of Metatranscriptomics in Symbiotic Associations Between Plants and Rhizosphere Microorganisms
Abstract
Interaction between plant and microbes in the rhizosphere, the place of soil influenced with the aid of plant roots, are fundamental to biogeochemical cycling, plant immunity, and productivity. These interactions are properly understood, however, exceedingly little is about the plant microbiome. The study of the interactions between plants and their microbial communities in the rhizosphere is important for developing sustainable management practices and agricultural products such as biofertilizers and biopesticides. Plant roots release a broad variety of chemical compounds to attract and select microorganisms in the rhizosphere. Rhizosphere symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. Understanding this symbiotic relationship at a molecular level provides important contributions to the understanding of forest ecosystems and global carbon cycling. Metatranscriptomics allowed the profiling of different microorganism communities and their evaluation of relative and quantitative profusion and metabolism from large number of samples. Extraction and purification of mRNA immediately from plant, decomposition of natural material and soil, accompanied with pooling of expressed genes by using high throughput sequencing, have spawned metatranscriptomics a new rising area of research. Every metatranscriptome offers a view of relative abundance and composition of genes which are actively transcribed and consequently provides the evaluation about the interaction between plant and soil microbes Metatranscriptomics can also evaluate the collective metabolism pathways of microorganism in different environments.