Enzymatic treatment improves ACE-I inhibiton and antiproliferative potential of chickpea

Abstract

Chickpea seeds are the preferred source of proteins possessing health care functions in countries across the world. Study indicated the chickpea proteins as a promising center of bioactive peptides and open up new vista for food industry. Employing gastrointestinal enzyme alcalase,\xa0protein hydrolysates generated from 45 chickpea seed accessions were\xa0evaluated for angiotensin-I converting enzyme (ACE-I) inhibitory potential and antiproliferative influence. Alcalase at \xa01\xa0h of optimum hydrolysis produced bioactive peptides inhibiting the ACE-I activity. The accession BDN-9-3 gave highest ACE-I inhibitory activity with IC50 value of 22.43\xa0mg/ml. The protein hydrolysate of BDN-9-3 was further subjected to antiproliferative assessment against breast cancer cells MCF-7 and MDA-MB-231. The IC50 of BDN-9-3 alcalase hydrolysate was 0.60\xa0mg/ml and 0.63\xa0mg/ml in MCF-7 and MDA-MB-231 cells respectively, compared to\xa0non hydrolyzed chickpea protein (IC50 of 0.85 and 0.82\xa0mg/ml). Present study ascertain that chickpea seed hydrolysate can be perceived as a valuable nutraceutical resource.