Expression patterns of natriuretic peptides in pre-hibernating and hibernating Anatolian ground squirrel (Spermophilus xanthoprymnus) kidney

Abstract

Hibernation is characterized by marked suppression of renal function. Natriuretic peptides (NPs) are involved in the regulation of renal function. However, the role of NPs in the renal function during hibernation remains unclear. We aimed to investigate the distribution patterns of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) in Anatolian ground squirrel (Spermophilus xanthoprymnus) kidneys during pre-hibernation and hibernation periods. Cortical proximal tubules showed weak ANP immunoreactivity, with moderate staining on the brush border during the pre-hibernation period. In the hibernation period, moderate ANP immunoreactivity was seen in cortical proximal tubules, with very weak reaction in hibernating cortical distal tubules, medullary proximal and collecting tubules. Cortical proximal and distal tubules of both periods had strong and weak BNP immunoreactivity, respectively. Medullary proximal, distal and Henle’s loop segments showed very weak BNP immunoreactivity during pre-hibernation. Medullary distal, proximal and collecting tubules and Henle’s loop segments had moderate staining during hibernation. In both periods, cortical proximal tubules displayed strong immunoreactivity to CNP. Distal tubules had moderate CNP staining during pre-hibernation, albeit weak staining during hibernation. Medullary proximal tubules exhibited moderate to strong immunoreactivity during pre-hibernation. Medullary distal and proximal tubules had weak and moderate CNP staining, respectively, during pre-hibernation. In both periods, Henle’s loop segments displayed moderate CNP immunoreactivity. Glomeruli had similar weak ANP, BNP and CNP staining in both periods. These results suggest that heterothermic conditions differently affected the expression of NPs in the squirrel kidney. This different expression of NPs may contribute to the renal adaptation during hibernation.