Silicon | 2019
Loading Metformin/Nettle Extract Lamium album L. subsp. Crinitum in Porous Hollow Silica Nanoparticle Coated by the Layer-by-Layer Method
Abstract
Metformin (MF) and L. albuman are used to treat type 2 diabetes. Diabetes causes problems such as blindness and albumin excretion. In this study, metformin, L. albuman and a composition of both were loaded onto porous hollow silica nanoparticles and synthesized by the sol-gel method. After the nanoparticles (NPs) were functionalized by (3-Aminopropyl) triethoxysilane (APTES), NPs were coated by chitosan (Ch) and alginate (Alg) polymers using the layer-by-layer (LBL) method. Optimized conditions including the amount of drug, amount of NPs and reaction time were studied. Finally, the percentage of the loading of the three drugs and their result was measured and compared. The morphology of the synthesized NPs was studied by scanning electron microscopy (SEM), X-Ray diffraction (XRD) was applied to characterize the crystalline structure of NPs, Brunauer-Emmett-Teller (BET) was used to measure the surface area of hollow NPs, Fourier Transform infrared (FTIR) spectroscopy was applied to porous loading of the drug into NPs, Zeta Potential analysis was used to measure the potential of NP layers and optimized conditions of loading three drugs and their rescuer was measured by UV-Visible spectroscopy. MTT assay was also used to study the toxicity of metformin loaded into hollow porous silica nanoparticles. The results of this study revealed that in optimized conditions 95%, 66% and 86% of metformin, L. album and a composition of both were loaded in NPs, respectively. Otherwise, 83%, 76% and 89% of them are released in the buffer solution with pH\u2009=\u20095.3 in 7\xa0h.