Colorimetric Detection of Glucose Using WO3 Nanosheets as Peroxidase-mimetic Enzyme

Abstract

In the present work, WO3 nanosheets(WO3 NSs) were prepared by a facile method at room temperature. The obtained WO3 NSs showed peroxidase-like activity, which could catalyze 3,3’,5,5’-tetramethylbenzidine(TMB) to form a blue oxidation product(ox TMB) in the presence of H2O2. Based on this, convenient and sensitive colorimetric methods for the detection of H2O2 and glucose were established. The linear ranges for detecting H2O2 and glucose were 1–200 µmol/L and 1–100 µmol/L, respectively. The limits of the detection of H2O2 and glucose were as low as 0.79 and 0.96 µmol/L, respectively. This method was also successfully applied to the detection of glucose in urine samples. The detection result was consistent with that of the value detected by the clinical method, indicating the potential in clinical diagnosis and biomedical detection.