Membraneless reproducible MoS2 field-effect transistor biosensor for high sensitive and selective detection of FGF21

Abstract

Fibroblast growth factor 21 (FGF21) serves as an essential biomarker for early detection and diagnosis of nonalcoholic fatty liver disease (NAFLD). It has received a great deal of attention recently in efforts to develop an accurate and effective method for detecting low levels of FGF21 in complex biological settings. Herein, we demonstrate a label-free, simple and high-sensitive field-effect transistor (FET) biosensor for FGF21 detection in a nonaqueous environment, directly utilizing two-dimensional molybdenum disulfide (MoS2) without additional absorption layers. By immobilizing anti-FGF21 on MoS2 surface, this biosensor can achieve the detection of trace FGF21 at less than 10 fg mL−1. High consistency and satisfactory reproducibility were demonstrated through multiple sets of parallel experiments for the MoS2 FETs. Furthermore, the biosensor has great sensitivity to detect the target FGF21 in complex serum samples, which demonstrates its great potential application in disease diagnosis of NAFLD. Overall, this study shows that thin-layered transition-metal dichalcogenides (TMDs) can be used as a potential alternative platform for developing novel electrical biosensors with high sensitivity and selectivity.摘要成纤维细胞生长因子21(FGF21) 是一种用于早期检测和诊断 非酒精性脂肪肝病(NAFLD)的必需生物标志物.最近,开发在复杂\xa0生物环境中准确有效地检测血液中低浓度FGF21的方法受到了极\xa0大的关注.本文展示了一种无标记、简单和高灵敏度的场效应晶\xa0体管 (FET)生物传感器,用于在非水环境中检测FGF21. 通过对二硫\xa0化钼(MoS2)表面进行功能化将抗FGF21牢固地固定在MoS2材料上,\xa0使该生物传感器实现检测FGF21的检测限小于10 fg mL−1.多组平\xa0行实验证明了MoS2 FET 的高度一致性和令人满意的再现性.此外,\xa0生物传感器对复杂血清样品中的目标FGF21具有很高的敏感度,这\xa0表明其在NAFLD疾病诊断中具有巨大的潜在应用前景.