Polymer and lipid-based nanoparticles to deliver RNAi and CRISPR systems

Abstract

Abstract The development of nano delivery vectors for plant genetic transformation needs more research for determining the delivery challenges and increasing plant genetic efficiency. Efficient in vivo delivery of the CRISPR/Cas9 system to the target cell—avoiding side effects and delivery barriers that conflict with other transport approaches—remains a challenge. Recently, nonviral vectors have emerged as new classes of gene carriers in terms of safety, simplicity, and adaptability. RNAi and CRISPR systems can be delivered by nonviral vectors that are more easily degraded in vivo, and the expression of this transition facilitates use in medicine, biology, genetics, and agriculture. Polymer and lipid nanoparticles can be produced more easily and the size, composition, and structure of inorganic nanoparticles can be precisely controlled. In this chapter, we present the latest research on RNAi and CRISPR delivery technology based on lipids and polymers that are produced through synthetic techniques.