Archives of Cardiovascular Diseases Supplements | 2021
In vivo and ex vivo longitudinal follow-up of resveratrol supplementation or restauration of a normal diet in female rat hearts submitted to high-fat-high-sucrose diet
Abstract
Introduction Prediabetic women are at greater risk of cardiovascular diseases than men, investigating new sex dependent strategic therapies is then essential to limit cardiovascular complications in prediabetic women. Objective We aimed to evaluate the effects of resveratrol supplementation (RSV) or restauration of a standard diet on the heart of prediabetic female rats submitted to high-fat-high-sucrose diet (HFS). Methods Wistar female rats were divided in 4 groups fed for 5\xa0months with: normal or HFS diet (CTRL/HFS), HFS with RSV (1\xa0mg/kg/day) during the last 2\xa0months or HFS for 3\xa0months and 2\xa0months of standard diet (RSD). We performed a longitudinal in vivo study of cardiac function, morphology and perfusion by MRI, then rats underwent an IPGTT. Ex vivo experiments on isolated perfused hearts were realized to study cardiac function [rate pressure product (RPP), end diastolic pressure (EDP)] and energy metabolism with 31P magnetic resonance spectroscopy during an ischemia-reperfusion injury (IR). Tissues were collected for analyzes. Results Five months of HFS diet-induced glucose intolerance (P\xa0 Conclusion The two approaches have distinct effects on cardiac function and energy metabolism of prediabetic female rats. Further studies are on the go to explore the mechanisms involved.