Twenty-six years of chemical fertilization decreased soil RubisCO activity and changed the ecological characteristics of soil cbbL-carrying bacteria in an entisol

Abstract

Abstract Soil autotrophic bacteria are important for sequestrating atmospheric CO2 and thus contribute to the global carbon cycle. However, information is limited on how chemical fertilization can alter the composition of soil autotrophic bacteria. In this study, the cbbL gene (a key gene in the Calvin-Benson-Bassham cycle that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, RubisCO) was used to study the abundance and composition of autotrophic bacteria in an entisol with 26\u202fyears of continuous fertilization. In this process, five chemical fertilization regimes were examined: without fertilization control (CT), nitrogen and phosphorus fertilization (NP), N and potassium fertilization (NK), PK fertilization (PK), and NPK fertilization (NPK). The results showed that after the 26-year (1991–2017) period, the CT with a neutral pH (7.55) had a higher RubisCO activity than did the chemical fertilizer treatments, but the cbbL abundance was the lowest under CT. Soil RubisCO activity positively correlated with soil pH (R2\u202f=\u202f0.67, P