Characterization and anti-inflammation of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on LPS-induced RAW 264.7 cells.

Abstract

In this study, a polysaccharide (CGP-BG) was successfully produced from waste distillers grain by Chaetomium globosum CGMCC 6882. Chemical analysis demonstrated that CGP-BG contained 3.49 ± 0.51 % protein. The molecular weight of CGP-BG was 52.37 KDa, which was composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid in the molar ratio of 4.11:7.34:13.31:20.99:1.07:0.91:4.75:0.36. In vitro assay showed that CGP-BG had scavenging activities on hydroxyl radical, DPPH radical, superoxide anion, and ABTS radical in a dose-dependent manner. Meanwhile, CGP-BG significantly inhibited nitric oxide and pro-inflammatory cytokine (TNF-α, IL-6, and IL-1β) production in the lipopolysaccharide (LPS)-induced RAW 264.7 cells. Moreover, CGP-BG notably improved the antioxidant status of LPS-treated macrophages by enhancing superoxide dismutase, glutathione peroxidase, and antioxidant capacity and mitigating malondialdehyde. This work provided the basis for the application of CGP-BG as an anti-inflammatory and antioxidant agent.