Chemical Physics Letters | 2021
Tip-enhanced Raman spectroscopy of Aβ(1-42) fibrils
Abstract
Abstract Using tip-enhanced Raman spectroscopy (TERS) imaging with a lateral optical resolution of ~16\xa0nm, the heterogeneity of Aβ(1-42) fibrils implicated in Alzheimer’s disease is investigated. The amount and spatial distribution of TERS bands assigned to aromatic amino acid residues (histidine, tyrosine and phenylalanine) and to parallel β-sheet and random coil secondary structures suggest that both the surface and core of Aβ(1-42) fibrils are probed, confirm the twisted nature of the fibrils and reveal the presence of several β-sheet and disordered fibril regions with different hydrophilicity. This study completes and bears out literature TERS data on Aβ(1-42) amyloid fibrils.