Ultrasensitive electrochemiluminescence immunosensor with wide linear range based on a multiple amplification approach

Abstract

Abstract An ultrasensitive and highly selective electrochemiluminescence (ECL) immunosensor is proposed for the detection of prostate specific antigen (PSA) based on multiple amplification via glucose oxidase (GOx) and platinum nanoparticles (Pt NPs) conjugated with polyglutamic acid (PGA). The proposed immunosensor has a wide linear range (1.0\u202fpg/mL–150\u202fng/mL) and low detection limit (0.6\u202fpg/mL) for PSA detection, which is due in part to the excellent catalytic activity of the Ab2-GOx-PGA-Pt NPs probe toward the luminol ECL reaction.