Whole-genome sequencing exploitation analysis of non-Saccharomyces yeast Nakazawaea ishiwadae GDMCC 60786 and its physiological characterizations

Abstract

Abstract The genomic and metabolic features of non-Saccharomyces yeast Nakazawaea ishiwadae GDMCC 60786 were investigated using its genome and by analyzing the metabolites of carbon source fermentation. N. ishiwadae GDMCC 60786, which showed resistant to environmental stresses (ethanol, SO2, pH, and NaCl) and a high ethyl acetate-production ability, was classified into the Nakazawaea genus based on the 26S rRNA gene. Complete genome sequences of the strain were obtained using the PacBio single-molecule real-time sequencing platform. The genome size was about 12,088,154 bp with 33.69% GC content. Key enzymes of glycolytic and pyruvate metabolism were identified by genes function annotation. KEGG analysis revealed that this strain possesses phosphoglucomutase, phosphofructokinase, phosphoglycerate kinase, phosphoglycerate mutase, pyruvate decarboxylase, and pyruvate dehydrogenase. Furthermore, combined with fermentation data on various carbon sources (e.g., glucose, fructose, maltose, and sucrose) and different amounts of ethanol and acetic acid, it was found that monosaccharides were more suitable as the carbon source of this strain to produce ethyl acetate. Compared with the control group, 4% (v/v) ethanol was used as supplementary carbon source, the content of ethyl acetate was increased 4 times.