A sea anemone-inspired small synthetic peptide at sub-ppm concentrations enhanced biofilm mitigation

Abstract

Abstract A biocide enhancer is a chemical that can enhance the efficacy of a biocide or reduce its dosage. In this work, a novel peptide was tested as a biocide enhancer. A chemically synthesized 14-mer peptide (Peptide A), which was found non-biocidal, has an amino acid sequence derived from sea anemone Actinia equina that possesses a biofilm-free exterior. Peptide A at 180\xa0ppb (w/w) or 100\xa0nM enhanced 100\xa0ppm tetrakis hydroxymethyl phosphonium sulfate (THPS) against an oilfield biofilm consortium grown on C1018 carbon steel in an enriched artificial seawater medium. The combination of 100\xa0nM Peptide A + 100\xa0ppm THPS led to additional 2-log reduction, 1-log reduction, 1-log reduction in sessile sulfate reducing bacteria (SRB) cell count, sessile acid producing bacteria (APB) cell count, and sessile general heterotrophic bacteria (GHB) cell count, in comparison with the treatment using 100\xa0ppm THPS alone in a 14-day laboratory biofilm prevention test. The data in this work suggest that non-biocidal Peptide A is a promising biocide enhancer that should be further explored.