Formulation and optimization of lemon balm extract loaded azelaic acid-chitosan nanoparticles for antibacterial applications

Abstract

Abstract Herein, preparation of chitosan/azelaic acid (CsAz) nanoparticles, loaded with lemon balm extract (LBE), and optimization of the formulation by using a mathematical model is reported. The model was designed by experimental design technique (DOE) to evaluate the effect of weight ratio of chitosan to azelaic acid (Cs/Az), tripolyphosphate (TPP) concentration and LBE weight on the loading efficiency of lemon balm in the chitosan nanoparticles. Data analysis from DOE indicated that the Cs/Az ratio (p-value 0.3014) exhibited no significant effect on the loading efficiency. Meanwhile, TPP concentration (p-values 0.0021) and LBE weight (p-value