Journal of Photochemistry and Photobiology A-chemistry | 2019

Supramolecular off-on-off fluorescent biosensor for total Free thyroid hormones detection based on their differential binding with cucurbit[7]uril to fluorescent perylene derivative



Abstract A novel host-guest biosensor composed of perylene dye derivatives and cucurbit[7]uril [CB7] is established for the determination of total thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The sensor was based on the competitive binding of the hormones (T3 and T4) with cucurbit[7]uril [CB7] to the fluorescent perylene derivative, N,N′-di(2-(trimethylammoniumiodide)ethylene) perylenediimide (TAIPDI). The binding constants between T3 and T4 with TAIPDI were determined to be 3.34\u2009×\u2009104 and 1.7\u2009×\u2009105 M–1, respectively, while the binding between TAIPDI and CB7 was found to be 1.48\u2009×\u2009103 M−1. The assembled biosensor showed different signal between T3 and T4 in the range of 0–106 and 0–25\u2009μM and detection limit of 16.1 and 5.66\u2009μM, respectively. The proposed sensor not only differentiates between the T3 and T4\u2009hormones, but also accurately determines the total thyroid hormones, which is crucial for the hyperthyroidism clinical diagnosis. This strategy based on the use of fluorescent probe combined with macrocycle host is economical and tackle tedious and longtime procedures for similar methods.

Volume 382
Pages 111945
DOI 10.1016/J.JPHOTOCHEM.2019.111945
Language English
Journal Journal of Photochemistry and Photobiology A-chemistry

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