An electrochemiluminescent biosensor for noninvasive glucose detection based on cluster-like AuAg hollowed-nanoparticles

Abstract

Abstract A cluster-like AuAg alloy hollow-nanoparticles (HNPs), synthesized by facile one-pot co-reduction under the guidance of bovine serum albumin (BSA), enhanced the electrochemiluminescence (ECL) of luminol, thus to be served as the sensing matrix of the enzymatic glucose biosensor. The prepared HNPs were characterized by electronic microscopy, UV-Vis spectrometry, IR spectrometry, X-ray diffraction analysis, X-ray photoelectron spectroscopy and electrochemistry. Using hydrolyzed APTMS to connect AuAg HNPs on indium tin oxide (ITO) coated glass, the synergistic effect between two metals makes it had 20 multiple enhancement of ECL, and shows sensitive response toward reactive oxygen species. After loading GOD on this matrix to build the glucose sensor, it has a wide linear response range for the glucose from 5.0 μM to 1.0 mM with a detection limit as 0.40 μM. It can be applied for noninvasive detection of glucose with saliva as specimen, the results indicated the high-degree correlation of glucose contents between saliva and blood.