A photoelectrochemical immunosensor based on ReS2 nanosheets for determination of collagen III related to abdominal aortic aneurysm

Abstract

Abstract 3-Butyl-1-dodecyl-imidazolium bromide ionic liquid was successfully synthesized and used as an auxiliary reagent for the exfoliation of ReS2 bulk crystal to produce ReS2 nanosheets. The photocurrent response of ReS2 nanosheets was enhanced 1.5-fold as integration with ionic liquid. ReS2 nanosheets were employed as a photosensitive element to construct a photoelectrochemical platform for immobilizing collagen III antibodies. As a result, a photoelectrochemical immunosensor was fabricated for determining collagen III. The ionic liquid can accelerate the separation of electron-hole pairs generated from ReS2 nanosheets, and thus help to improve photoelectrochemical sensing characteristics. Some experimental conditions were optimized to provide satisfied signals for determining collagen III. Under optimized conditions, the photocurrent decline of the PEC immunosensor is linearly related to the logarithm of collagen III concentration in the range from 0.001 to 1000 ng mL-1 with a detection limit of 0.1 pg mL-1. The ReS2 based photoelectrochemical immunosensor has also been successfully used for assaying collagen III in clinical serum samples.