Chlorophyll nitrogen isotope values track shifts between cyanobacteria and eukaryotic algae in a natural phytoplankton community in Lake Erie

Abstract

Abstract Chlorophylls are produced by all photosynthetic organisms and are ideal targets for compound-specific isotopic studies of phytoplankton. In laboratory cultures, the difference between the nitrogen (N) isotope ratio (δ15N value) of chlorophyll and the δ15N value of biomass, known as epor, varies taxonomically, yielding potential applications for studying productivity in modern and ancient environments. Here we take advantage of the annual cyanobacterial bloom in Lake Erie, USA, to demonstrate epor patterns in a natural community. The resulting time series shows that environmental observations are similar to laboratory cultures: predicted epor endmember values range from 4.6‰ to 7.4‰ for eukaryotic algae, and −18‰ to −21‰ for cyanobacteria. Because the range and sensitivity of epor is similar between laboratory and natural settings, the data support the use of epor as a reliable tracer of the relative contributions of cyanobacteria and eukaryotic algae to nutrient utilization and primary production in lacustrine environments.