Enhanced activity and modified substrate-favoritism of Burkholderia cepacia lipase by the treatment with a pyridinium alkyl-PEG sulfate ionic liquid

Abstract

Abstract Three types of pyridinium salts, i.e., 1-ethylpyridin-1-ium cetyl-PEG 10 sulfate (PY ET ), 1-butylpyridin-1-ium cetyl-PEG 10 sulfate (PY BU ), and 1-(3-methoxypropyl)pyridin-1-ium cetyl-PEG 10 sulfate (PY MP ), have been prepared and evaluated for their activation property of Burkholderia cepacia lipase by comparison to the control IL-coated enzymes, 1-butyl-2,3-dimethylimidazolium cetyl-PEG 10 sulfate-coated lipase PS (IL1-PS). Among the tested pyridinium salt-coated lipases, the PY ET -coated lipase PS (PY ET -PS) exhibited the best results; the transesterification of 1-(pyridin-2-yl)ethanol, 1-(pyridin-3-yl)ethanol, 1-(pyridin-4-yl)ethanol, or 4-phenylbut-3-en-2-ol proceeded faster than those of the IL1-PS-catalyzed reaction while maintaining an excellent enantioselectivity (E\u202f>\u202f200). This improved efficiency was found to be dependent on the increased K cat value.