American Journal of Obstetrics and Gynecology | 2019
Performance of open two‐port fetoscopic fetal spina bifida repair in a newly developed program: 207
Abstract
206 Visible light-cured glycol chitosan (GC) bioadhesive hydrogel for fetoscopic prenatal treatment of myelomeningocele Hyun Sun Ko, Dae Hyeok Yang, Jeong Ha Wie, Ahyoung Kim, Heung Jae Chun, Jong Chul Shin Catholic University of Korea, Seoul, Seoul, Korea (Republic of), Catholic University ok Korea, Seoul, Seoul, Korea (Republic of) OBJECTIVE: Myelomeningocele (MMC) is a congenital disease which can leave irreversible neurological sequelae after birth. Due to the significant maternal and fetal complication of open fetal surgery, tissue engineering with fetoscopic approach has the potential to provide a less invasive approach for fetal therapy. Our study focuses on the development and characterization of the physical and biological properties of visible light-cured glycol chitosan (GC) hydrogels, which is applicable in wet condition without suture or glue, for fetoscopic MMC prenatal repair. STUDY DESIGN: Visible light-cured GC hydrogels were prepared and characterized. Then it was applied prenatally to retinoic acidinduced fetal MMC in the rat model. RESULTS: The results demonstrate that the GC hydrogel is nontoxic, can support cellular migration, can be applicable in wet condition, and can be adhered by visible light, without suture or glue. When GC hydrogel is applied prenatally to retinoic acid-induced fetal MMC in the rat model, without suture or glue, epidermal ingrowth and neovascularization were significantly increased by hydrogels, compared to the nontreatment group. CONCLUSION: The characteristics of visible light-cured GC hydrogels, which is applicable in wet condition without suture or glue, make it a potential novel alternative to open fetal repairs.