Induced refractive error changes optical coherence tomography angiography transverse magnification and vascular indices.

Abstract

PURPOSE\nTo assess the effect of changing anterior eye refractive power with contact lenses on the transverse magnification of enface images and associated vascular indices from optical coherence tomography angiography (OCT-A).\n\n\nDESIGN\nProspective cross-over study.\n\n\nMETHODS\nSpherical soft contact lenses (-6D to +6D in 2D steps) were used to induce anterior eye refractive power changes in 11 healthy young adults and 3\u202f×\u202f3mm macular scans were captured using OCT-A (Zeiss AngioPlex, software version 11.0; Cirrus HD-OCT 5000, Carl Zeiss Meditec Inc.). Image transverse magnification was predicted based on refraction and biometry measurements and compared with empirical changes in the enface images measured with image analysis. Linear regression analysis was performed to assess the relationship between induced refractive ametropia and foveal avascular zone (FAZ) area, perimeter, circularity and vessel density and perfusion density.\n\n\nRESULTS\nThe predicted transverse magnification was linearly related to induced refractive ametropia and to the empirical transverse magnification changes (average slope:1.02,95%CI:0.90-1.34). All the OCT-A indices showed linear relationships with induced refractive ametropia (p<0.05) with the 12D tested range altering the indices by 7-12%. After correcting for transverse magnification, all OCT-A indices except FAZ area were linearly related to induced refractive ametropia (p<0.05) and were reduced to 1-9%.\n\n\nCONCLUSIONS\nThis study is the first to show that induced refractive ametropia can affect OCT-A image magnification and indices. These changes are clinically important and need to be considered along with biometry effects when interpreting OCT-A indices. Transverse magnification changes can affect the ability of OCT-A to precisely measure linear dimensions of blood vessels.