Detection and quantification of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in bacteremia induced by interdental brushing in periodontally healthy and periodontitis patients.

Abstract

OBJECTIVE\nThis study was aimed to compare the presence and amounts of bacteremia induced by interdental brushing in periodontally healthy (H) and periodontitis (P) individuals using culture based (direct culture [DC]) and molecular based techniques (real-time quantitative polymerase chain reaction [qPCR]) in a cross-sectional study model.\n\n\nMATERIALS AND METHODS\nAfter a full mouth periodontal evaluation, blood samples were taken before and 1\u2009min after professionally-administered interdental brushing. These samples were analyzed by DC and qPCR, targeting Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Subgingival samples were also collected and analyzed. Student t-test, chi-squar tests and correlations were used for analyzing the data.\n\n\nRESULTS\nThirty individuals per group were included. P. gingivalis and A. actinomycetemcomitans were detected with qPCR methods, but not with DC. At baseline, bacteremia was observed in 5\u2009P patients (16.7%) and in 2 H individuals (6.6%) (p\u2009=\u20090.421). After interdental brushing, bacteremia was only observed in 2\u2009P patients (6.6%) (p\u2009=\u20090.901). A positive correlation between subgingival and blood levels of A. actinomycetemcomitans was observed (r\u2009=\u20090.3; p\u2009=\u20090.013).\n\n\nCONCLUSION\nBacteremia related to A. actinomycetemcomitans and P. gingivalis did not significantly increase after a single session of use of interdental brushes.