Bioresource technology | 2019
Polyhydroxy butyrate production by Acinetobacter junii BP25, Aeromonas hydrophila ATCC 7966, and their co-culture using a feast and famine strategy.
Abstract
The study aimed to evaluate biopolymer production using two bacterial strains, Acinetobacter junii BP25 and Aeromonas hydrophila ATCC 7966, and their co-culture. Batch experiments were evaluated using acetate and butyrate as carbon sources in feast and famine strategy. Feast phase was studied using carbon, nitrates and phosphate in the ratio of 100:8:1 and famine phase was limited with the phosphate and nitrates. Co-culture resulted in highest specific growth rate (0.30\u202fh-1) in the feast phase and the famine phase accounted the maximum polyhydroxybutyrate (PHB) accumulation (2.46\u202fg PHB/L), followed by Acinetobacter junii BP25 (0.25\u202fh-1 and 1.82\u202fg PHB/L) and Aeromonas hydrophila ATCC 7966 (0.17\u202fh-1 and 1.12\u202fg PHB/L). Fourier-transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR) structural analysis confirmed as PHB. PHB production using the co-culture could be integrated with biohydrogen process using volatile fatty acids (VFA) as a carbon source in the biorefinery framework.