Colloids and surfaces. B, Biointerfaces | 2021
One-step self-assembly of magnetic supramolecular metal-organic coordination functionalized MoS2 complex as nanoenzyme-reactor.
Abstract
In the present study, a kind of magnetic supramolecular metal-organic coordination complex (SMOCC) functionalized MoS2 was prepared with one-step in aqueous solution for enzyme immobilization. As possessing a protective nanocoating of PDA/PEI/Cu2+ (polydopamine: PDA, polyethyleneimine: PEI), the proposed material can provide biocompatible microenvironment and flexible adhesion force on particle interface, which is conductive to loading laccase (170.0\u2009±\u20091.8\u2009mg/g) with high activity (93.0\u2009±\u20091.1 %). Compared with the free laccase, the immobilized laccase has higher stability in a broader range of pH (3-10), temperature (20-80\u2009°C), storage time (1-18 days) and reusability (1-16 cycles). The removal of carcinogenic persistent organic pollutant malachite green in the water with the immobilized laccase shows a higher efficiency (89.4\u2009±\u20091.2 %) than free laccase (16.2\u2009±\u20090.2 %). The Fe3O4@MoS2@(PDA/PEI/Cu2+) nanocomposites can also be used successfully to immobilize trypsin, lipase and catalase respectively, showing a satisfactory enzyme loading (157.0\u2009±\u20090.1\u2009mg/g, 151.6\u2009±\u20091.4\u2009mg/g, 162.6\u2009±\u20091.6\u2009mg/g, respectively) and activity (95.0\u2009±\u20090.5 %, 90.0\u2009±\u20090.8 %, 91.0\u2009±\u20090.9 %, respectively). The MoS2 can be replaced by carbon material and similar results can be obtained.