Effects of exogenous 3-indoleacetic acid and cadmium stress on the physiological and biochemical characteristics of Cinnamomum camphora.

Abstract

Indoleacetic acid (IAA) is a plant growth regulator that plays an important role in plant growth and development, and participates in the regulation of abiotic stress. To explore the effect of IAA on cadmium toxicity in Cinnamomum camphora, an indoor potted experiment was conducted with one-year-old C. camphora seedlings. The influence of IAA on cadmium accumulation, net photosynthetic rates, respiration, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids), osmoregulatory substances (proline, soluble sugar and soluble protein) and the malondialdehyde content in C. camphora leaves treated with 30\xa0mg kg-1 cadmium was analysed with or without the addition of 10\xa0mg kg-1 IAA. Cadmium accumulation in the leaves of C. camphora with the addition of exogenous IAA was significantly higher than accumulation during cadmium stress without additional IAA (ca 69.10% after 60 days incubation). During the culture period, the net photosynthetic rate in C. camphora leaves subjected to cadmium stress without the addition of IAA was up to 24.31% lower than that of control plants. The net photosynthetic rate in C. camphora leaves subjected to cadmium stress and addition of IAA was up to 30.31% higher than that of leaves subjected to cadmium stress without the addition of IAA. Chlorophyll a, total chlorophyll and carotenoid contents in the cadmium-stressed leaves without the addition of IAA were lower than those in the control treatment. The presence of IAA increased the chlorophyll a, total chlorophyll and carotenoid contents relative to the cadmium stress without the addition of IAA. The respiration rate and concentrations of proline, soluble sugar, soluble protein and malondialdehyde in C. camphora leaves subjected to cadmium stress without the addition of IAA were higher than those in the control. The addition of IAA reduced the respiration rate, and the concentrations of proline, soluble sugar, soluble protein and malondialdehyde in C. camphora leaves when compared with the cadmium stress without the addition of IAA. These results indicate that exogenous IAA improves photosynthetic performance and the growth environment of C. camphora by enhancing the net photosynthetic rate, increasing concentrations of osmoregulatory substances, removing reactive oxygen radicals and eliminating potential damage, thereby reducing the toxic effects of cadmium on C. camphora.