An investigation on in vitro anti-inflammatory and antiproliferative potential of isolated Labdane diterpenoids from Andrographis paniculata (Burm. f.) wall. Ex nees: An important medicinal plant prescribed in Ayurveda

Abstract

Abstract Introduction The inflammatory response is a highly regulated process, and its dysregulation can lead to the establishment of chronic inflammation and in some cases, to death. Diterpenoids have found to be an important class of phytochemicals possessing anti-inflammatory potential. Methods In present study, some Labdane diterpenoids isolated from Andrographis paniculata like, Andrographolide (compound A); 14-deoxy-11,12-didehydroandrographolide (compound B); 14-Deoxyandrographolide (compound C) and 3,14- Dideoxyandrographolide (compound D) were evaluated for their cyclooxygenase inhibitory, antioxidant and anti-proliferative activity using different cancer cell lines. Results The results obtained revealed that compound C has maximum inhibitory potential towards COX-2 (83.24%; IC50 9.0\u202fμg/ml). The docking data was found to be in accordance with the in vitro experimental results, with compound C demonstrating the promising binding free energy (-8.1 Kcal/mol). The anti-proliferative data showed that all compounds had significant cytotoxicity towards selected (HCT-15, INT- 407 and HeLa-B75) cancer cell lines, while compounds also demonstrated effective antioxidant potential. The antioxidant profile reveals that, the compound C possess most promising SOR scavenging activity (91.41\u202f±\u202f1.56%), while the lowest activity was recorded from compound D (16.30\u202f±\u202f0.25). The results obtained by in silico molecular docking study are in accordance with the in vitro activities. Conclusion The isolated compounds showing promising anti-inflammatory and antiproliferative activity could be considered for acceleration in research for development of diterpenoids based anti-inflammatory agents.