Analysis of added phosphates in hake fillets by ion-exchange chromatography: A case study of false positives induced by nucleotides coelution.

Abstract

This study aimed to optimize an ion-exchange chromatography method for an accurate quantification of phosphates. Coelution of nucleotides was detected following the quantification of small amounts of di- and trimetaphosphates in hake fillets. In some samples, triphosphates were added to the glaze ice, therefore, defrosting waters (glaze ice\xa0+\xa0drip loss of fillets) should be analyzed for an effective assessment of the use of phosphates in seafood. Even low amounts of adenosine 5 -diphosphate (ADP) and inosine 5 -monophosphate (IMP) triggered false positive results for P2O74- and P3O93-. Keeping the initial isocratic conditions for longer was crucial for the separation of ADP from P2O74- and of IMP from P3O93-. The improved method showed that in general, hake fillets did not have diphosphates, and trimetaphosphates were residual. Triphosphates were clearly added in only four defrosting waters, and in most of the remaining ones, diphosphates and trimetaphosphates contents corresponded to false positive results.