Reactive oxygen species, aging and articular cartilage homeostasis

Abstract

Abstract Chondrocytes are responsible for the maintenance of the articular cartilage. A loss of homeostasis in cartilage contributes to the development of osteoarthritis (OA) when the synthetic capacity of chondrocytes is overwhelmed by processes that promote matrix degradation. There is evidence for an age‐related imbalance in reactive oxygen species (ROS) production relative to the anti‐oxidant capacity of chondrocytes that plays a role in cartilage degradation as well as chondrocyte cell death. The ROS produced by chondrocytes that have received the most attention include superoxide, hydrogen peroxide, the reactive nitrogen species nitric oxide, and the nitric oxide derived product peroxynitrite. Excess levels of these ROS not only cause oxidative‐damage but, perhaps more importantly, cause a disruption in cell signaling pathways that are redox‐regulated, including Akt and MAP kinase signaling. Age‐related mitochondrial dysfunction and reduced activity of the mitochondrial superoxide dismutase (SOD2) are associated with an increase in mitochondrial‐derived ROS and are in part responsible for the increase in chondrocyte ROS with age. Peroxiredoxins (Prxs) are a key family of peroxidases responsible for removal of H2O2, as well as for regulating redox‐signaling events. Prxs are inactivated by hyperoxidation. An age‐related increase in chondrocyte Prx hyperoxidation and an increase in OA cartilage has been noted. The finding in mice that deletion of SOD2 or the anti‐oxidant gene transcriptional regulator nuclear factor‐erythroid 2‐ related factor (Nrf2) result in more severe OA, while overexpression or treatment with mitochondrial targeted anti‐oxidants reduces OA, further support a role for excessive ROS in the pathogenesis of OA. Therefore, new therapeutic strategies targeting specific anti‐oxidant systems including mitochondrial ROS may be of value in reducing the progression of age‐related OA. Graphical abstract Figure. No caption available. HighlightsAn age‐related increase in chondrocyte reactive oxygen species (ROS) levels exists.Mitochondrial dysfunction contributes to oxidative stress in osteoarthritis.Excessive ROS levels alter signaling to promote cartilage matrix destruction.