Comparison of two DNA extraction methods: PrepFiler® BTA and modified PCI-silica based for DNA analysis from bone

Abstract

Abstract Bones are important evidence often found in crime scene associated with mass grave disaster and missing persons. Substantial fragmentation and intermixing of bones limit the use of morphological observation. Human individualization using DNA analysis can complement but the challenges are the PCR inhibitors contained and low amount of DNA in degraded bone. The selection of effective DNA extraction method that minimizes PCR inhibitors and maximizes DNA recovery thus is crucial. This study aimed to compare the efficiency of the currently modified PCI-silica and the latest forensic commercial kit developed for challenging bone sample (PrepFiler® BTA). The results showed that PrepFiler® BTA provided significantly higher median DNA concentration (p