Characterization and immune function of the thioredoxin-interacting protein (TXNIP) from Litopenaeus vannamei.

Abstract

The thioredoxin (Trx) system plays essential roles in maintenance and regulation of the redox state of cysteine residues in cellular proteins. The Trx-interacting protein (TXNIP) is a TRX inhibitory protein that works as a negative regulator in the TRX system. The function of TXNIP in invertebrates, in particular in immunity, remains unclear to date. In the current study, a novel TXNIP from Pacific white shrimp Litopenaeus vannamei was identified and characterized and its roles in immune responses was investigated. TXNIP could interact with Trx and inhibit its redox regulatory activity, suggesting that TXNIP was involved in regulation of the cellular redox state in shrimp. The expression of TXNIP was high in the stomach, gill, scape, eyestalk, epithelium, pyloric and muscle and low in the hepatopancreas, intestine, nerve, hemocytes and heart. Stimulations with pathogens white spot syndrome virus (WSSV) and Vibrio parahaemolyticus and immune stimulants poly (I:C) and LPS could significantly increase the expression of TXNIP in vivo. Silencing of TXNIP using RNAi strategy significantly facilitated the infection of V. parahaemolyticus but inhibited the infection of WSSV in shrimp. These indicated that TXNIP could be positively involved in antibacterial responses but negatively involved in antiviral responses in shrimp. Moreover, knockdown of TXNIP in vivo exerted opposite effects on expression of antimicrobial peptides anti-lipopolysaccharide factors and penaeidins and enhanced the phagocytic activity of hemocytes against bacteria. These suggested that TXNIP could play a complex role in regulation of humoral and cellular immune responses in shrimp.