Glucocorticoid receptor in ayu (Plecoglossus altivelis): Genomic and non‐genomic effects on monocytes/macrophages function

Abstract

ABSTRACT The glucocorticoid receptor (GR) is an important feedback regulator of the hypothalamic‐pituitary‐interrenal (HPI) axis. However, there are a limited number of studies focused on host‐pathogen interactions in which an association between GR and immune response has been evaluated in monocytes/macrophages (MO/M#) after being challenged with highly pathogenic bacteria. Here, we cloned the cDNA sequence of the glucocorticoid receptor (PaGR) gene from ayu fish. The PaGR transcript was expressed in all tissues, and changes in expression were observed in immune tissues and MO/M# after live Vibrio anguillarum infection. Subsequently, PaGR was expressed and purified to prepare anti‐PaGR antibodies. We analyzed the subcellular localization of PaGR. PaGR was expressed not only in the intracellular space but also in the plasma membrane. PaGR activation decreased the expression of pro‐inflammatory cytokines and increased the expression of anti‐inflammatory cytokines. However, PaGR activation suppressed the phagocytosis activity of V. anguillarum‐infected ayu MO/M# via a non‐genomic pathway. Interestingly, PaGR activation could enhance MO/M# bacterial killing capability and apoptosis. Therefore, PaGR may modulate the immune response in ayu MO/M# by genomic and non‐genomic pathways. HighlightsWe characterized a GR gene from ayu, Plecoglossus altivelis.GR transcripts were altered in ayu upon Vibrio anguillarum infection.GR activation suppressed the phagocytosis of moncytes/macrophages via non‐genomic pathway.GR participated in the regulation of apoptosis in moncytes/macrophages.