Chemo-profiling of bioactive metabolites from Chaetomium globosum for biocontrol of Sclerotinia rot and plant growth promotion.

Abstract

Chaetomium globosum Kunze ex. Fries has been known to produce diverse bioactive metabolites, attracting researchers to exploit the biocontrol agent for plant disease management. However, distinct research gaps are visible regarding detail characterization of bioactive metabolites. Thus the current study has been planned to characterize volatile and nonvolatile compounds of most potential strain of C.\xa0globosum 5157. GC-MS analysis of hexane fraction revealed twenty-six volatile organic compounds, representing 65.5% of total components in which 3-octanone (21.4%) was found to be most abundant. UPLC-QTOF-MS/MS analysis of ethyl acetate and methanolic fractions resulted tentative characterization of fifteen and eleven metabolites, respectively. Among these, nine metabolites were isolated, purified and characterized using 1H NMR and High resolution mass spectrometric analysis to delineate mass fragmentation pattern for the first time. Antifungal potential of hexane fraction exhibited high inhibitory action against Sclerotium rolfsii (139.2\xa0μg\xa0mL-1) whereas ethyl acetate fraction was highly effective against Sclerotinia sclerotiorum (112.1\xa0μg\xa0mL-1). Comparative assessment of C.\xa0globosum 5157 vis a vis Trichoderma harzianum A28 revealed promising effect of C.\xa0globosum 5157 with respect to antifungal properties and plant growth promotion of Brassica seedlings.