Production of pectin lyase from agricultural wastes by isolated marine Penicillium expansum RSW_SEP1 as dye wool fiber

Abstract

Enzyme technology is considered one of the most promising tools in the field of biotechnology as applied biocatalysts. The marine fungal strain Penicillium expansum RSW_SEP1 isolated from red sea water at Sharm El-Shiekh province and identified by 18S rRNA gene (accession number MH754656). The strain was able to produce pectin lyase from different agricultural wastes as the cheapest and most economical carbon source. The maximal enzyme activity of 88.83 U/mL was obtained from the pea peel (Pp) and sugar cane bagasse (Scb) at ratio 5:5 (w/w) and pH 6 by 8 mL of spore suspension as inoculum. Different concentrations of pectin lyase were examined on green dyed. The highest value of color strength (K/S) at 15% of wool fibers dyed with green algae by microwave after 5 min of the pretreated wool at pH 5.0. Different concentrations % of pectin lyase was examined on green dyed. The results indicated that, the highest value of color strength (K/S) at 15% of wool fibers dyed with green algae by microwave after 5 min of the pretreated wool at pH 5.0. This study focused on isolation and molecular identification of marine fungal strain as pectin lays producer from agriculture wastes and its application of dyeing wool fibers.