International Journal of Antimicrobial Agents | 2021
Clinical characteristic and outcome of Severe Acute Respiratory Infection (SARI) cases in a tertiary hospital, Malaysia: A descriptive study
Abstract
withdrawn PTM-002 Mycobacterium species on the cutaneous microbiome of very preterm neonates admitted to the NICU in a tertiary health care hospital Pritik A. Shah, Varun Govindarajan, Ambica Rangaiah, Shivshankar Diggikar, Sahana Devadas, Sneha K. Chunchanur, Sathyanarayan Muthur Shankar, Mallesh Kariyappa. Department of Microbiology, Bangalore Medical College and Research Institute, India; Department of Pediatrics, Bangalore Medical College and Research Institute, India; Department of Neonatology, OvumWoman and Child Specialty Hospital, Bengaluru, India Introduction: The neonatal skin microbiome consists of all the genomes and genetic products of microorganisms harbouring on the skin of babies. This is mainly constituted by bacteria of phyla Actinobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Tenericutes. Neonatal microbiota helps in angiogenesis, immune function, intestinal T‐cell development, gut‐associated lymphoid tissue development, etc. Host and the microbiota develop a harmonious environment resulting in symbiosis. Any disruption of this environment could lead to pathological disease. Methods: The study was conducted to understand the neonatal skin microbiome of very preterm neonates admitted to Neonatal Intensive Care Unit at a tertiary health care setting before and after KangarooMother Care. The study included collection of skin swabs of preterm neonates (n-30) before 32 weeks of gestation and admitted to a Level III NICU in a Tertiary Health Care Hospital. The first swab (Sample A) was collected between the 5th–10th day of postnatal life. A second swab (Sample B) was taken after 7 days of adequate KMC (minimum 6 hours per day). The swabs were sent to the genomics laboratory in sterile water for Next-generation sequencing (NGS). Detection was done using Credence Rapid Infection Detection (credence RIDTM) for Next Generation Sequencing after Polymerase Chain Reaction (PCR). Ethical approval was obtained for the study from Institutional Ethics Committee (IEC BMCRI/PS/184/2020–21). Results: Data obtained was in the form of relative abundance with respect to the presence of other organisms in the given clinical isolate. In sample A, the observed average relative abundance for M. tuberculosis was identified to be 0.013904093% present in 83.33% (25/30) of samples and for M. abscessus 0.116270902% present in 100% (30/30) of samples, while in sample B the values observed for M. tuberculosis was 0.01142986% in 66.67% (20/30) and for M. abscessus was 0.100288231% in 93.33% (28/30) of samples. We foundMycobacterium tuberculosis in 83.33% & 66.67% (p-value, 0.285) and Mycobacteroides abscessus in 100% & 93.33% Abstracts from the 13th ISAAR 2021 / International Journal of Antimicrobial Agents 58S1 (2021) 106421