Cosmetic potentials of extracts and compounds from Zingiber cassumunar Roxb. rhizome

Abstract

Abstract Zingiber cassumunar Roxb. is a medically important plant with extensive traditional medicinal functions and bioactivities. It is also used in some skin-care balm by local people. Nevertheless, the research on the active components of the plant related to skin-care is not enough, which mainly involved some antioxidant and anti-inflammatory active compounds. This study investigated the extracts and components from rhizome of Z. cassumunar for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, HDFa collagen secretion promotion, tyrosinase inhibition, and NO generation inhibition activities for the purpose of identifying ingredients with potential anti-aging, skin-whitening, and anti-inflammation for use in cosmetics. Ethyl acetate (EtOAc) extract of the plant showed significant NO generation inhibition, DPPH radical scavenging, tyrosinase inhibitory activities, and weak promoting collagen secretion activity while petroleum ether (PE) extract showed obvious NO generation inhibition activity. Six active compounds, (E)-3-(3,4-dimethoxyphenyl)-2-propenal (1), cis-3-(3,4-dimethoxyphenyl)-4-[(E)-2,4,5-trimehoxystyryl]cyclohex-1-ene (2), 1-feruloyloxy cinnamic acid (3), (1E,4E,6E)-1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (4), bisdemethoxycurcumin (5), and curcumin (6), were isolated from PE and EtOAc extracts by bioassay-guided fractionation. 1, 3, 4, 5, and 6 showed significant NO generation inhibition activity with IC50 of 22.67\u202f±\u202f2.36, 7.00\u202f±\u202f0.22, 15.40\u202f±\u202f0.63, 5.47\u202f±\u202f0.09, and 7.16\u202f±\u202f0.05\u202fμM, respectively. 3 also showed potent DPPH radical scavenging activity with IC50 of 22.96\u202f±\u202f0.87\u202fμg/mL. 4 was a good tyrosinase inhibitor with the inhibitory rate of 42.56\u202f±\u202f1.02% under the concentration of 100\u202fμg/mL. 2 and 4 showed significant HDFa collagen secretion promoting activity with EC50 of 13.91\u202f±\u202f1.56 and 8.50\u202f±\u202f1.31\u202fμg/mL, respectively. This is the first report on components from Z. cassumunar for their HDFa collagen secretion promotion and tyrosinase inhibition activities. These results suggest that medicinal plant Z. cassumunar possesses potential application value in the development of natural cosmeceutical products for anti-aging, skin-whitening, and anti-inflammation activity.