Mixed cellular inflammation is increased in adult asthma with severe chronic rhinosinusitis: 576

Abstract

Michael Goldberg, MD PhD, Hadar Gershon, Michael Appel, PhD, Liat Nachshon, MD, Michael B. Levy, MD FAAAAI, Ilan Youngster, Arnon Elizur, MD, and Omry Koren; Institute of Allergy Immunolgy & Pediatric Pulmonology, Zeriffin, Israel, Bar Ilan University, Safed, Israel, Assaf Harofeh Medical Center, Zerifin, Israel, Assaf-Harofeh Medical Center, Meitar, Israel, Assaf Harofe Medical Center, Zerifin, Israel, Institute of Allergy, Immunology & Pediatric Pulmonology, Zeriffin, Israel. RATIONALE: Changes to the gut microbiota have been associated with allergies to individual foods. However, whether an intersecting common dysbiotic state exists in IgE-mediated food allergy (FA) across the major allergenic food types, is unclear. Our objective was to identify a gut microbial signature associated with IgE-mediated FA, and to determine whether unique clusters exist for each of the allergenic foods studied. METHODS: Fecal samples were collected from 309 allergic patients (86 milk, 85 peanut, 89 treenut, 37 sesame, 12 egg), and an age-matched control group (n540). Gut microbiome was profiled by bacterial 16S rRNA sequencing on an Illumina MiSeq platform and microbiome analyses performed using QIIME2. Alpha (within sample) and Beta (between sample) diversity were calculated. RESULTS: A preliminary analysis was performed on approximately half of the samples obtained. The microbiome composition of allergic patients was significantly different compared to age-matched controls. Beta diversity differences were observed (p<0.001), indicating the presence of a potential gut microbiota signature in IgE-mediated food allergy (Figure 1). Allergic patients had significantly reduced Bacteroidetes and Proteobacteria, and a higher abundance of Firmicutes and Actinobacteria, as compared to age-matched controls. PCoA of samples via weighted and unweighted UniFrac revealed that treenuts allergic group was significantly more similar to one another than was the milk allergic group (p<0.001). CONCLUSIONS: These results suggest that patients with persistent IgEmediated FA have a specific microbiota signature. The role of altered input from both the allergic state and the particular nutritional deficits associated with each food needs to be explored. Y 576 Mixed cellular inflammation is increased in adult asthma with severe chronic rhinosinusitis