Skeletal Muscle Fibre Type Changes in an Avian Model of Hepatic Fibrosis.

Abstract

We investigated the susceptibility of type I and type II skeletal myofibres to atrophy in hens with hepatic fibrosis induced by bile duct ligation (BDL). Seven hens, approximately 2 years old, were randomly assigned to BDL (n\xa0=\xa04) and sham surgery (SHAM) (n\xa0=\xa03) groups. Mean body weight and mean liver weight as a percentage of mean body weight were significantly lower in the BDL group than in the SHAM group at 4 weeks post surgery (P\xa0=\xa00.002, P\xa0=\xa00.005, respectively). Mean plasma aspartate aminotransferase activity was slightly higher, while total cholesterol (P\xa0<0.001), total bilirubin (P\xa0=\xa00.022) and NH3 (P\xa0=\xa00.048) concentrations were\xa0significantly higher in the BDL group than in the SHAM group. Liver lesions were induced in all hens in the BDL group. The weights of the pectoralis (PCT) (P\xa0=\xa00.049) and flexor perforans et perforatus digiti III (FPPD III) muscles (P\xa0=\xa00.006) as a percentage of body weight were significantly decreased in the BDL group. A significantly reduced mean myofibre cross-sectional area in the PCT of BDL hens (P\xa0=\xa00.005) was indicative of atrophy. No significant differences were observed in the fibre type composition of the PCT, supracoracoideus or FPPD III muscles between the SHAM and BDL groups. However, there was an approximate 43% increase in the number of type I fibres in the femorotibialis lateralis of the BDL group and small angular type II fibres and large round type I fibres in this muscle were characteristic of peripheral neuropathy. The results suggest that type II fibres are more susceptible to atrophy than type I fibres in this model of hepatic fibrosis.