Electropolymerization of molecularly imprinted polypyrrole film on multiwalled carbon nanotube surface for highly selective and stable determination of carcinogenic amaranth

Abstract

Abstract A novel amaranth electrochemical sensor was produced by electrodepositing a molecularly imprinted polypyrrole film in the presence of amaranth on a multi-walled carbon nanotube (MWCNT) surface. Template molecules were completely eluted through an innovative strategy that only requires the application of a fixed electrical potential in a blank phosphate buffer solution. Obviously, this strategy is much faster and more eco-friendly. The molecularly imprinted polymer (MIP)-based electrochemical sensor was systematically investigated by scanning electron microscopy, atomic force microscopy, X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. The resultant MIP/MWCNT modified glass carbon electrode (MIP/MWCNT/GCE) shows satisfactory electroactive surface area and charge transfer resistance. As expected, the electrochemical response signals were significantly amplified at the MIP/MWCNT/GCE. Under optimal conditions, the proposed MIP/MWCNT/GCE exhibits extraordinarily good analytical performance for amaranth detection, with two wide detection ranges (0.007\xa0μM to 1.0\xa0μM, and 0.40\xa0μM to 17\xa0μM) and detection possible down to 0.4\xa0nM. Moreover, the polypyrrole MIP films show high binding affinity and specifically identifies amaranth molecules, enabling the robust detection of amaranth in a complicated matrix with excellent selectivity, reproducibility, and stability. The proposed sensor achieves accurate detection of amaranth concentration in various fruit drinks with acceptable recovery.